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Coverage Report

  1. Project Clover database Wed Nov 13 2024 16:12:26 GMT
  2. Package jalview.ext.htsjdk

File VCFReader.java

 

Coverage histogram

../../../img/srcFileCovDistChart8.png
21% of files have more coverage

Code metrics

28
46
12
1
254
143
29
0.63
3.83
12
2.42

Classes

Class Line # Actions
VCFReader 37 46 29
0.7558139675.6%
 

Contributing tests

This file is covered by 6 tests. .

Source view

1    /*
2    * Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
3    * Copyright (C) $$Year-Rel$$ The Jalview Authors
4    *
5    * This file is part of Jalview.
6    *
7    * Jalview is free software: you can redistribute it and/or
8    * modify it under the terms of the GNU General Public License
9    * as published by the Free Software Foundation, either version 3
10    * of the License, or (at your option) any later version.
11    *
12    * Jalview is distributed in the hope that it will be useful, but
13    * WITHOUT ANY WARRANTY; without even the implied warranty
14    * of MERCHANTABILITY or FITNESS FOR A PARTICULAR
15    * PURPOSE. See the GNU General Public License for more details.
16    *
17    * You should have received a copy of the GNU General Public License
18    * along with Jalview. If not, see <http://www.gnu.org/licenses/>.
19    * The Jalview Authors are detailed in the 'AUTHORS' file.
20    */
21    package jalview.ext.htsjdk;
22   
23    import java.io.Closeable;
24    import java.io.File;
25    import java.io.IOException;
26   
27    import htsjdk.samtools.util.CloseableIterator;
28    import htsjdk.variant.variantcontext.VariantContext;
29    import htsjdk.variant.vcf.VCFFileReader;
30    import htsjdk.variant.vcf.VCFHeader;
31    import jalview.bin.Console;
32   
33    /**
34    * A thin wrapper for htsjdk classes to read either plain, or compressed, or
35    * compressed and indexed VCF files
36    */
 
37    public class VCFReader implements Closeable, Iterable<VariantContext>
38    {
39    private static final String GZ = "gz";
40   
41    private static final String TBI_EXTENSION = ".tbi";
42   
43    private static final String CSI_EXTENSION = ".csi";
44   
45    private boolean indexed;
46   
47    private VCFFileReader reader;
48   
49    /**
50    * Constructor given a raw or compressed VCF file or a (csi or tabix) index
51    * file
52    * <p>
53    * If the file path ends in ".tbi" or ".csi", <em>or</em> appending one of
54    * these extensions gives a valid file path, open as indexed, else as
55    * unindexed.
56    *
57    * @param f
58    * @throws IOException
59    */
 
60  6 toggle public VCFReader(String filePath) throws IOException
61    {
62  6 indexed = false;
63  6 if (filePath.endsWith(TBI_EXTENSION)
64    || filePath.endsWith(CSI_EXTENSION))
65    {
66  0 indexed = true;
67  0 filePath = filePath.substring(0, filePath.length() - 4);
68    }
69  6 else if (new File(filePath + TBI_EXTENSION).exists())
70    {
71  0 indexed = true;
72    }
73  6 else if (new File(filePath + CSI_EXTENSION).exists())
74    {
75  0 indexed = true;
76    }
77   
78    /*
79    * we pass the name of the unindexed file to htsjdk,
80    * with a flag to assert whether it is indexed
81    */
82  6 File file = new File(filePath);
83  6 if (file.exists())
84    {
85  6 reader = new VCFFileReader(file, indexed);
86    }
87    else
88    {
89  0 Console.error("File not found: " + filePath);
90    }
91    }
92   
 
93  5 toggle @Override
94    public void close() throws IOException
95    {
96  5 if (reader != null)
97    {
98  5 reader.close();
99    }
100    }
101   
102    /**
103    * Returns an iterator over VCF variants in the file. The client should call
104    * close() on the iterator when finished with it.
105    */
 
106  1 toggle @Override
107    public CloseableIterator<VariantContext> iterator()
108    {
109  1 return reader == null ? null : reader.iterator();
110    }
111   
112    /**
113    * Queries for records overlapping the region specified. Note that this method
114    * is performant if the VCF file is indexed, and may be very slow if it is
115    * not.
116    * <p>
117    * Client code should call close() on the iterator when finished with it.
118    *
119    * @param chrom
120    * the chromosome to query
121    * @param start
122    * query interval start
123    * @param end
124    * query interval end
125    * @return
126    */
 
127  40 toggle public CloseableIterator<VariantContext> query(final String chrom,
128    final int start, final int end)
129    {
130  40 if (reader == null)
131    {
132  0 return null;
133    }
134  40 if (indexed)
135    {
136  0 return reader.query(chrom, start, end);
137    }
138    else
139    {
140  40 return queryUnindexed(chrom, start, end);
141    }
142    }
143   
144    /**
145    * Returns an iterator over variant records read from a flat file which
146    * overlap the specified chromosomal positions. Call close() on the iterator
147    * when finished with it!
148    *
149    * @param chrom
150    * @param start
151    * @param end
152    * @return
153    */
 
154  40 toggle protected CloseableIterator<VariantContext> queryUnindexed(
155    final String chrom, final int start, final int end)
156    {
157  40 final CloseableIterator<VariantContext> it = reader.iterator();
158   
159  40 return new CloseableIterator<VariantContext>()
160    {
161    boolean atEnd = false;
162   
163    // prime look-ahead buffer with next matching record
164    private VariantContext next = findNext();
165   
 
166  77 toggle private VariantContext findNext()
167    {
168  77 if (atEnd)
169    {
170  0 return null;
171    }
172  77 VariantContext variant = null;
173  179 while (it.hasNext())
174    {
175  145 variant = it.next();
176  145 int vstart = variant.getStart();
177   
178  145 if (vstart > end)
179    {
180  6 atEnd = true;
181  6 close();
182  6 return null;
183    }
184   
185  139 int vend = variant.getEnd();
186    // todo what is the undeprecated way to get
187    // the chromosome for the variant?
188  139 if (chrom.equals(variant.getContig()) && (vstart <= end)
189    && (vend >= start))
190    {
191  37 return variant;
192    }
193    }
194  34 return null;
195    }
196   
 
197  76 toggle @Override
198    public boolean hasNext()
199    {
200  76 boolean hasNext = !atEnd && (next != null);
201  76 if (!hasNext)
202    {
203  40 close();
204    }
205  76 return hasNext;
206    }
207   
 
208  37 toggle @Override
209    public VariantContext next()
210    {
211    /*
212    * return the next match, and then re-prime
213    * it with the following one (if any)
214    */
215  37 VariantContext temp = next;
216  37 next = findNext();
217  37 return temp;
218    }
219   
 
220  0 toggle @Override
221    public void remove()
222    {
223    // not implemented
224    }
225   
 
226  86 toggle @Override
227    public void close()
228    {
229  86 it.close();
230    }
231    };
232    }
233   
234    /**
235    * Returns an object that models the VCF file headers
236    *
237    * @return
238    */
 
239  4 toggle public VCFHeader getFileHeader()
240    {
241  4 return reader == null ? null : reader.getFileHeader();
242    }
243   
244    /**
245    * Answers true if we are processing a tab-indexed VCF file, false if it is a
246    * plain text (uncompressed) file.
247    *
248    * @return
249    */
 
250  0 toggle public boolean isIndex()
251    {
252  0 return indexed;
253    }
254    }